The Metafer module Metafer Metaphase automatically finds metaphases on suitable samples. Due to user-trainable classifiers it is easily adaptable to all kinds of preparations and contrasting methods (Giemsa staining, fluorescence, and even phase contrast). Detected metaphases are automatically displayed in an overview gallery and can be relocated with just one mouse click. Coordinates are easily transferred to manual microscopes. The direct interface to workstations with Ikaros installed makes metaphase finding with Metafer Metaphase a powerful tool for convenient and fast chromosome analysis.

Easy adaptation and parameter optimization to different cell types and preparations is of crucial importance for making an automated scanning system useful for routine analysis. To facilitate this task, Metafer Metaphase provides sophisticated training and optimization routines. The metaphase gallery, generated in real time during the search, provides fast overview of detected objects. Metaphases are easily relocated by clicking on the respective gallery image to allow for detailed inspection under the microscope, or using the digital live image. With the mouse, metaphases can easily be selected for acquisition, or they can be rejected or deleted in the gallery. Alternatively, the search classifier can be trained to assign quality scores to the metaphases, allowing for automatically selecting a defined number of best metaphases.

Additional parameters such as the mitotic index (MI) are automatically obtained during the scan. Positions of all metaphases and nuclei are displayed, and metaphases can be selected interactively or automatically. If clonal samples are scanned, the Metafer Metaphase can even select a given number of objects per clone, and it assigns the clone number to the metaphase's metadata.

Automated acquisition of high-resolution metaphase images efficiently increases the degree of automation in routine metaphase analysis. Each scanning system operated by Metafer generates position lists, representing the location of all detected objects. These positions are automatically relocated, and images of the objects at the position are acquired based on classifier-defined parameters. Once acquired, images are immediately available to any connected workstation.

The way how the images are acquired can be highly customized. After metaphase finding, for instance, all detected objects can automatically be sorted by their quality score. Metafer Metaphase can acquire a pre-defined number of best metaphases. To provide a complete picture, however, some randomly selected metaphases can additionally be captured. Objects can be acquired as raw images, or they can be subject to image processing. Focus stacks are supported as well as up to 12 different color channels, for example to automatically acquire FISH or mFISH metaphases. Objects are automatically centered in the image, and if they are larger than one camera field it is possible to acquire more than one image per position.

The automated immersion oil dispenser (AutoOiler) eliminates any human interaction between metaphase finding and image acquisition. Thus, if the SlideFeeder x80 is installed, the integrated scanning system managed by Metafer can find and capture images from up to 800 slides without any need for supervision by a researcher. Therefore, the integrated scanning system can capture images through the nights and over weekends or holidays.