About 100 guests from 36 countries met on the XVIII. MetaSystems Distributor Meeting (DM) in November to exchange experiences and to get to know new trends and developments at MetaSystems.
Does your application not match any of the above categories? MetaSystems may still be able to find a solution for your imaging problem.
MetaSystems devices excel through a unique combination of flexibility and reliability. This makes it easy to adapt a MetaSystems product like the Metafer slide scanning platform to new applications. Below you will find several success stories - maybe your application can also be automated?
Microscopic analysis of thin mineral sections is a common tool to evaluate the micro-texture and structure of rocks. Characteristics observed under the microscope include color and color variations under polarized light (pleochroism). Using the manifold possibilities of Metafer1, and the microscope features for polarized light, it is possible to create a system for automated imaging of thin rock sections.
To visualize RNA expression in Drosophila larvae, the central imaging facility of a large biotechnology lab wanted to combine Nomarski phase contrast images with fluorescence. Since Metafer1 has the flexibility to support any contrasting method the microscope offers, it was easily possible to create an adapted workflow. The resulting images contain a color image of the Drosophila larvae acquired with Nomarski phase contrast and a fluorescence channel showing the spatial distribution of RNA expression in the larvae.
A few years ago, MetaSystems was approached by a renowned marine research institute. The request involved imaging, and identifying diatoms in samples obtained from the sea floor and other places. Diatoms, a major group of algae, are important indicators for environmental conditions. Diatoms are enclosed with a cell wall made of silica showing a large diversity in appearance. A Metafer1 system and an adapted viewer software were installed and configured to communicate with existing diatom identification software. Now, the high-quality diatom images from Metafer1 are used routinely as a basis for automated diatom detection and identification.
Arabidopsis thaliana is a 20-25 cm tall flowering plant native to Europe, Asia, and northwestern Africa with a rapid life cycle of six weeks. It is used extensively as a model organism in plant biology and genetics. With about 157 million base pairs and five chromosomes, Arabidopsis has one of the smallest genomes among plants, and it was the first one to be sequenced in 2000.
It is known that two A. thaliana genes, QRT1 and QRT2, are required for pollen separation during normal development. In certain mutants, however, pollen grains are released in tetrads. A tetrad is a cluster of 4 pollen grains that are the 4 products of one meiosis, which are still attached together. In a visual assay utilizing transgenic marker constructs, it is possible to encode pollen-expressed fluorescent proteins of three colors in the quartet mutant background. This bears the possibility to study the results of one single meiosis and to analyze the crossing over interference (inhibition of nearby crossing overs) based on the specific fluorescence color pattern of the tetrads.
After harvesting, the fluorescence of the reporter proteins in the tetrads vanishes within 4 hours. Therefore, the number of analyzed tetrads is limited if manual microscopy is used. An automated imaging protocol based on the Metafer1 slide scanning platform, is capable of finding and identifying tetrads on the sample automatically. Thus, far more tetrads can be analyzed in the time before the markers vanish. Analysis of the tetrads is also automated by classifying the different color patterns. All data is conveniently summarized in automated reports.