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Rare Events

Detection of Rare Cells

Fast and reliable detection of specifically labeled, rare objects on microscope slides.

The two main situations that take particular advantage of automatic slide scanning are rare event detection and statistical scoring of a large number of cells. The detection of fetal nucleated red blood cells (fNRBCs) in maternal blood presents an example of extremely rare events; another example would be the evaluation of circulating tumor cells (CTCs) for tumor staging and therapy monitoring. It is obvious that speed is essential in rare cell detection. The amazing speed of Metafer eliminates the need for enrichment steps with their inherent risk of losing cells of interest.

Fetal Cells

Isolating fetal nucleated red blood cells (fNRBCs) from the maternal blood facilitates prenatal genetic analysis without the need for diagnostic procedures that are invasive for the fetus. Such isolation usually involves specific antibody labeling and a technique for finding the extremely rare target cells in the sample. It could be shown that automated microscopy, the core skill of MetaSystems, is a feasible method for detecting fNRBCs. MetaSystems has developed elaborated algorithms to make extremely fast, yet precise detection of rare events possible.

For the detection of rare fetal cells, Metafer scans several thousands of cells per second. All detected objects are stored with their positions on the slide and a gallery image. The system detects rare fetal cells based on their staining pattern. Scanning is performed in the primary signal channel. Specific staining patterns such as ring-shaped, membrane bound antibody staining are precisely identified. A second signal color channel can be used for confirming the detected object. Additionally, counter stain signals are used to distinguish the finding from extracellular debris.

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Circulating Tumor Cells

Circulating tumor cells (CTCs) are cells that have wandered off from a primary tumor into blood or the lymphatic system and are transported around the body in circulation. CTCs are believed to be the origin of additional tumors (metastases) in distant organs, causing the vast majority of cancer-related deaths. It is consensus that detection of CTCs may have important prognostic and therapeutic implications.

Metafer's ability to detect rare events in fastest time makes it a perfect tool for finding CTCs. But the system can do much more than localizing the cells. Each detected object in Metafer is related to its position on the sample. Thus, a gallery of detected CTCs, for example, also resembles a position list for subsequent scans. It is therefore possible to let Metafer relocate the tumor cells, and to perform further automated tasks such as FISH signal analysis. With this ability, the identified cells can be investigated thoroughly to gain additional information on their origin.

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